Evaluation of Cell Toxicity Effect and Capacity of Free Radical Inhibitory by Methanol Extract of Johreniopsis stricticaulis on MCF-7 Cell Line in Pre and Past Flowering Stages

Introduction: Plants have a range of phenolic compounds that have antioxidant properties and these natural antioxidants reduce the risk of cancer by protecting cells and increasing the strength of antioxidants in the plasma. The aim of this study was evaluated cytotoxicity effects of methanol extract of Johreniopsis stricticaulis on MCF-7 cell line. Methods: After collection of Johreniopsis stricticaulis, the plant was dried and powdered. Methanol extract was obtained by maceration method. Antioxidant activity and total phenolic content were evaluated by DPPH and folin-ciocalteu methods; cytotoxic effect on MCF-7 was evaluated by MTT method. Results: The methanolic extract of the plant in the pre flowering stage had a higher phenol content and this extract had higher antioxidant activity. Cytotoxicity effect was measured at different times (24, 48, 72 hours); methanol extract in pre-flowering stage possessed no inhibitory effect on cell growth, but in past-flowering stage was effective (IC50 ≥ 300μg/ml, 234.1 ± 6.1, 246.3 ± 5.5). Conclusion: The results of this study showed that plant collection stage is effective on plant characteristics, this is due to the type of compounds in plant. As the plant passes from the flowering stage, some of the compounds may be destroyed. On the other hand, due to little cytotoxic effect on this cell line; so cytotoxic effect on other cell lines were recommended.